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FAST AND CHEAP PROTEIN PURIFICATION. Economical alternatives to conventional chromatography-based recombinant protein purification

Код 893370

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Аннотация к книге "FAST AND CHEAP PROTEIN PURIFICATION. Economical alternatives to conventional chromatography-based recombinant protein purification"

Two naturally produced in vivo affinity resin substitutes have been combined with a self-cleaving tag to develop two novel affinity-based purification technologies. The first resin alternative is polyhydroxybutyrate (PHB) granules and the second is elastin-like polypeptides (ELP). An engineered tripartite protein fusion is expressed in E. coli and purified through simple centrifugation. The intein self-cleaving tag then releases the target protein with a mild pH shift. The two systems have been...

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Издательство: Книга по требованию
Дата выхода: июль 2011
ISBN: 978-3-6391-5443-6
Объём: 136 страниц
Масса: 227 г
Размеры(высота, ширина, толщина), см: 23 x 16 x 1

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