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MRNA display

Код 2542924

  • ISBN: 978-5-5127-9190-5

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Аннотация к книге "MRNA display"

High Quality Content by WIKIPEDIA articles! mRNA display is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a puromycin linkage. The complex then binds to an immobilized target in a selection step (affinity chromatography). The mRNA-protein fusions that bind well are then reverse transcribed to cDNA and their sequence amplified via a polymerase chain reaction. The end result is a nucleotide sequence that encodes a peptide with high affinity for the molecule of interest.

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ISBN: 978-5-5127-9190-5

Книга находится в категориях

Биология Математика Домашние животные Путешествия, туризм, природа

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