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SSCP Conditions Optimization. Optimization of Single Strand Conformational Polymorphism (SSCP)Conditions for Exon 4-12 of LDLR Gene

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Аннотация к книге "SSCP Conditions Optimization. Optimization of Single Strand Conformational Polymorphism (SSCP)Conditions for Exon 4-12 of LDLR Gene"

Single strand conformational polymorphism (SSCP) is a technique which is used to detect point mutation in genes. The purpose was to optimize the condition of SSCP for efficient and sensitive mutation detection in Exon 4, 5, 6, 7, 8, 9, 10, 11 and 12. All these Exon were amplified and run on agarose gel to check strong and specific amplification. These samples were then subjected for vertical electrophoresis for SSCP using different conditions of gel concentration, time, temperature and voltage...

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Издательство: Книга по требованию
Дата выхода: июль 2011
ISBN: 978-3-8433-7297-8
Объём: 68 страниц
Масса: 123 г
Размеры(высота, ширина, толщина), см: 23 x 16 x 1

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