Crude Lysate
Frederic P. Miller, Agnes F. Vandome, John McBrewster
High Quality Content by WIKIPEDIA articles! High Quality Content by WIKIPEDIA articles! A crude lysate is the solution produced when cells are destroyed by disrupting their cell membranes, often with detergent or other chaotropic agent, in a process known as cytolysis. This releases the contents within the cell. Crude cell lysates are routinely produced in biochemistry and cell biology laboratories during the process of protein purification, although purified cellular organelles can also be retrieved from the solution. After a crude lysate has been generated, the first step in processing a crude lysate is often ultracentrifugation, which separates the solution into distinct bands containing organelles, membrane lipids, proteins, and...
ISBN: 978-6-1340-2671-0
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Книга по требованию
Дата выхода: июль 2011