Effect of Plant Extracts on RNA Expression Profiles. Expression...
Ruzaidi Azli Mohd Mokhtar, Valentino Milton Junior Gumbilai, Wilson Yong Thau Lym
Diabetes is a major public health problem. The new therapies developments that are able to improve glycemia management, cure diabetes and even protect from it are of great interest. This study investigated the effect of selected plant extracts on the Insulin 2 gene expression profile against STZ- induced pancreatic cell damages. M.charantia extract showed the highest value of total phenolic content, antioxidant activity and total flavanoid content compared to M. citrifolia and P. speciosa extracts. Expression of both GAPDH as the reference gene and Ins2 gene as the target gene was measured by SYBR Green I fluorescence; melting curves and agarose gel electrophoresis were used to verify the specificity of gene (Ins2) for diabetic rats...
ISBN: 978-3-8443-0172-4
Издательство:
Книга по требованию
Дата выхода: июль 2011