Transfer RNA Dynamics in Bacterial Protein Synthesis. Studies of...
Dongli Pan
Protein biosynthesis, also called translation, refers to mRNA-programmed synthesis of a polypeptide on the ribosome. tRNAs are required to move efficiently between several positions on the ribosome during the elongation phase of translation. Although structures of tRNA bound to ribosomes are available, the dynamics and mechanism of tRNA movement and structural change are incompletely understood. The classic L-shape of tRNA undergoes considerable distortion during the elongation cycle, leading to the suggestion that the tRNA tertiary core region, which stabilizes the L-shape, actively interacts with the ribosome. Here, using fluorescence labeling of tRNA and mutagenesis of highly conserved nucleotides within the tertiary core, combined...
ISBN: 978-3-6390-8503-7
Издательство:
Книга по требованию
Дата выхода: июль 2011